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. 2021 Jul 27;12:704958. doi: 10.3389/fpls.2021.704958

FIGURE 6.

FIGURE 6

Genetic and functional interactions of AIF2 with BIN2 and BZR1 in the regulation of seed size and weight. (A,B) Evaluation of genetic and functional interaction of AIF2 with BIN2 in the regulation of seed size (A) and seed weight (B). Transgenic constructs ectopically expressing AIF2ox or AIF2dC were transformed into a BIN2 gain-of-function mutant, bin2-1, or a BIN2 loss-of-function mutant, bin2bil1bil2 (bin2KO), and their seed phenotypes were evaluated. (C,D) Evaluation of genetic and functional interaction of AIF2 with BZR1 in the regulation of seed size (C) and seed weight (D). Transgenic constructs ectopically expressing AIF2ox or AIF2dC were transformed into a BZR1 gain-of-function mutant, bzr1-1D, or plants constitutively expressing bzr1-1DdEAR, a loss-of-function form of BZR1, were crossed to aif2-1 to examine their seed phenotypes. Statistical difference between bracketed samples is indicated by an asterisk (*) at P < 0.05 and two asterisks (**) at P < 0.01. Number of seeds examined for measurement of length/width ratio and weight >800. Scale bars in pictures represent 0.5 mm in length.