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. 2021 Jul 27;12:704958. doi: 10.3389/fpls.2021.704958

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Copyright © 2021 Kim, Kim, Shin and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reduced starch and oil accumulation in seeds of AIF2-overexpressing p35S:AIF2-EGFP (AIF2ox) transgenic plants. (A) Photographic comparison of starch accumulation in developing seeds. Siliques were stained with Lugol’s staining dye, and seeds with embryos at the same developmental stage were compared for starch accumulation. Number of seeds examined for each stage of the corresponding plants >150. Pictures show a representative image. (B,C) Total RNA was extracted from siliques with developing seeds, and the transcript expression for sucrose transport-related genes (B) and lipid biosynthesis genes (C) was measured using qRT-PCR. Relative expression of each gene is normalised as a ratio of the Col-0 plants, which was set to 1. Statistical difference from the Col-0 control is indicated by two asterisks (^∗∗) at P < 0.01.