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. 2021 Jul 27;12:704958. doi: 10.3389/fpls.2021.704958

FIGURE 8.

FIGURE 8

Sucrose- and brassinosteroid-induced suppression of AIF2 stability and silique phenotypes. Flowers in between DAP3.5 to DAP4 (n > 40 for each experiment) were collected and cultured for 9 days in solid MS media containing brassinolide (BL, 10– 9 M) supplemented with or without 3% sucrose (w/v). (A) Length and seed numbers of in vitro-cultured silique. (B) Western blot analysis of AIF2-EGFP expression in siliques of in vitro-cultured p35S:AIF2-EGFP transgenic lines (AIF2ox) (C,D) Total RNA was extracted from in vitro-cultured siliques of the AIF2ox, and the transcript expression for lipid biosynthesis genes (C) and sucrose transport-related genes (D) was measured using qRT-PCR. Relative expression of each gene is normalised as a ratio of the mock-treated plants, which was set to 1. Statistical difference from the mock-treated control is indicated by an asterisk (*) at P < 0.05 and two asterisks (**) at P < 0.01.