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. 2021 Aug 5;2(3):100715. doi: 10.1016/j.xpro.2021.100715
Reagent Amount/Sample Storage temperature (°C)
Buffer X (Neural Tissue Dissociation kit (P)) 2,850 μL 4°C
Enzyme P (Neural Tissue Dissociation kit (P)) 75 μL −20°C
Buffer Y (Neural Tissue Dissociation kit (P)) 30 μL 4°C
Enzyme A (Neural Tissue Dissociation kit (P)) 15 μL −20°C
Myelin Removal Beads II 200 μL 4°C
Red Blood Cell Lysis Solution 500 μL 4°C
CD11b MicroBeads mouse/human 10 μL 4°C
Binding Buffer Stock Solution (Dead Cell Removal Kit) 250 μL 4°C
Dead Cell Removal MicroBeads (Dead Cell Removal Kit) 100 μL 4°C