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. 2021 Jul 27;12:709467. doi: 10.3389/fphar.2021.709467

FIGURE 4.

FIGURE 4

Chronic Elabela infusion regulates the renin-angiotensin-aldosterone system in heart and kidneys of salt-loaded hypertensive SHR. (A,B), quantitative RT-PCR analysis of RAAS genes. mRNA levels of angiotensin converting enzyme (ACE), angiotensin converting enzyme 2 (ACE2), ratio ACE/ACE2, and neprilysin (Mme) in heart (A) and kidneys (B) of SHR rats fed with standard-(SHR) or high-salt diet (8% of NaCl) and treated with either Apelin-13 (SHR-Salt-Ape13), Elabela (SHR-Salt-ELA) or saline (SHR-Salt). The Ribosomal protein L30 (Rpl30) of heart and kidneys was used as a reporter gene and the data normalized to SHR rats fed with standard-salt diet (0.3% of NaCl). All data are individual values with means ± SEM. *p < 0.05, **p < 0.01, the data were analyzed with one-way ANOVA and Bonferroni’s post hoc test (SHR n = 5, SHR-Salt n = 6, SHR-Salt-Ape13 n = 6, and SHR-Salt-ELA n = 6); (C), cleavage assay of 1 mM of Ape13 or ELA with 50 nM of purified rat angiotensin converting enzyme (ACE), angiotensin converting enzyme 2 (ACE2) or neprilysin (NEP). All values are means ± SEM of three independent experiments.