TABLE 3.

AMR gene profile of the 10 strains.

		Strains
		Reference	3	12	18	57	61	100A	240	274	285	298
Gene	Mutation
gyrA	S91F/Y	S	S	F	F	F	S	F	F	S	F	S
	D95N/G/A	D	D	A	A	A	D	A	D	D	A	D
parC	E91K/G	E	E	G	E	E	E	E	G	E	G	G
ponA1 (pbp1)	L421P	L	P	L	P	P	L	P	P	L	P	L
rpsJ	V57M	V	M	M	M	M	M	M	M	M	M	M
penA/pbp2	D345 insertion	–	D	D	D	D	D	D	D	D	D	D
	F504L	F	L	L	L	L	L	L	L	L	L	L
	A510V	A	V	V	V	V	V	V	V	V	V	V
	A516G	A	G	G	G	G	G	G	G	G	G	G
	P551S/L	P	P	P	S	S	P	S	L	S	S	S
porB1b (penb)	G120D/K	G	G	G	D	D	G	D	D	G	G	G
	A121D	A	A	A	D	G	A	G	A	S	S	G
	N122	N	N	N	N	D	N	S	N	K	K	N
pilQ (penC)	QAATPAKQ insertion at 180	–	–	–	–	–	–	–	–	QAATPAK	–	–
	D526N	D	D	D	D	D	D	D	D	D	G	D
	N640S	N	N	N	N	N	N	N	N	N	S	N
folP	T66M	T	M	T	T	T	T	T	M	T	M	T
	R228S	R	S	S	S	S	S	S	S	S	S	S
mtrR	A39T	A	T	T	T	T	T	T	A	A	A	A
	H105	H	H	H	H	H	H	H	Y	Y	Y	H
rplD	G70D/S/A/R	G	D	Y	G	Y	G	G	G	G	G	G
rpoB	H552N	H	N	H	N	N	H	N	H	H	H	H
Ciprofloxacin resistance		–	–	R	R	R	I	I	–	–	R	R
Gentamycin resistance		–	–	I	–	–	–	–	–	–	–	–

Mutations observed in the studied strains have been listed, mutated residues are indicated in bold, and mutations identified in this study have been underlined. The resistance profile for the strains as inferred through MIC has been indicated for ciprofloxacin and gentamycin. The isolates were sensitive to the other antibiotics that were tested as inferred from Table 2. The newly identified mutations need to be experimentally investigated for their role in antibiotic resistance.