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. 2021 Jul 14;297(2):100966. doi: 10.1016/j.jbc.2021.100966

Figure 3.

Figure 3

m971 epitope delineation on CD22.A, CDR loops from m971 interacting with the CD22 d7 domain (wheat). CDR loops from the variable heavy chains (deep teal) HCDR1, HCDR2, and HCDR3 interact with β-strand C. LCDR1 and LCDR2 (cyan) interact with loops C–E and E–F of d7. B, binding affinity of m971 Fab to CD22 ECD bearing complex N-linked glycans from expression in HEK 293F cells (CD22F), more homogeneous and high-mannose glycans from expression in HEK 293S cells (CD22S), and CD22 ECD containing the N634A mutation to remove this N-linked glycosylation site (CD22N634A). KD’s are indicated with the SEM and derive from two independent measurements. Each symbol represents a specific monosaccharide: N-acetylglucosamine (blue rectangle), fucose (red triangle), mannose (green circle), galactose (yellow circle), and Neu5Ac (purple diamond). CDR, complementarity-determining region; ECD, extracellular domain; HCDR, heavy-chain complementarity-determining region; LCDR, light-chain complementarity-determining regions.