| Reagent | Stock concentration | Final concentration | Amount |
|---|---|---|---|
| Tris-HCl (pH7.5) | 1 M | 20 mM | 1 mL |
| LiCl | 8 M | 1 M | 6.25 mL |
| EDTA | 500 mM | 2 mM | 200 μL |
| Nuclease-free water | n/a | n/a | 42.55 mL |
| Total | n/a | n/a | 50 mL |
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