Reagent | Stock concentration | Final concentration | Amount |
---|---|---|---|
Tris-HCl (pH7.5) | 1 M | 10 mM | 500 μL |
LiCl | 8 M | 150 mM | 937.5 μL |
500 mM EDTA | 500 mM | 1 mM | 100 μL |
Nuclease-free water | n/a | n/a | 48.5 mL |
Total | n/a | n/a | 50 mL |
Reagent | Stock concentration | Final concentration | Amount |
---|---|---|---|
Tris-HCl (pH7.5) | 1 M | 10 mM | 500 μL |
LiCl | 8 M | 150 mM | 937.5 μL |
500 mM EDTA | 500 mM | 1 mM | 100 μL |
Nuclease-free water | n/a | n/a | 48.5 mL |
Total | n/a | n/a | 50 mL |