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. 2021 Aug 6;2(3):100724. doi: 10.1016/j.xpro.2021.100724
Reagent Stock concentration Final concentration Amount
Tris-HCl (pH7.5) 1 M 10 mM 500 μL
LiCl 8 M 150 mM 937.5 μL
500 mM EDTA 500 mM 1 mM 100 μL
Nuclease-free water n/a n/a 48.5 mL
Total n/a n/a 50 mL