REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, buffers and enzymes
N6-methyladenosine (m6A)	Selleckchem	Cat#S3190
Adenosine	Sigma-Aldrich	Cat#A9251
10× Phosphate Buffered Saline (PBS)	Corning	Cat#46-013-CM
EDTA 0.5 M (pH8.0)	Promega	Cat#V4231
Glycerol	Sigma-Aldrich	Cat#G5516-100ML
LiCl 8 M	Sigma-Aldrich	Cat#L7026-100ML
MgCl2 25 mM	New England Biolabs (NEB)	Cat#B9021S
ZnCl2 0.1M	Sigma-Aldrich	Cat#39059-1ML-F
NaCl 5M	Quality Biological Inc	Cat#351-036-721EA
Ammonium Bicarbonate	Sigma-Aldrich	Cat#09830
Nuclease-free water	HyClone	Cat#SH30538.FS
200 Proof Ethanol	Sigma-Aldrich	Cat#459836
RNase decontamination solution	Genesee Scientific	Cat#10-456
PCR grade water	IBI Scientific	Cat#IB42301
Sodium acetate 3 M (pH5.2)	Corning	Cat#46-033-CI
Tris Hydrochloride (Tris-HCl) 1 M (pH7.5)	Fisher Scientific	Cat#BP1757-500
Beta-Mercaptoethanol	Sigma-Aldrich	Cat#M3148-25ML
Hydrochloric Acid (HCl) 6 M	Fisher Scientific	Cat# S25857
Nuclease P1 from Penicillium citrinum	Sigma-Aldrich	Cat#N8630-1VL
Alkaline phosphatase from Escherichia coli	Sigma-Aldrich	Cat#P5931-100UN
Oligo(dT)25 magnetic beads	New England Biolabs	Cat#S1419S
Water (HPLC Grade)	Fisher Scientific	Cat#AA22934M6
Acetonitrile (HPLC Grade)	Fisher Scientific	Cat#6000247
Ammonium Acetate	Spectrum Chemical	Cat#A2149-1KG
Ammonium Hydroxide	Spectrum Chemical	Cat#A1195
23G Syringe	Fisher Scientific	Cat#309571
1.5 mL low-bind tube	Corning	Cat#3207
Cell scraper	Corning	Cat#3008
0.2 μm PES filter	Whatman Puradisc	Cat#6780-2502
1 mL Syringe	Becton Dickinson	Cat#329654
Snap Cap, pre-slit	Fisher Scientific	Cat#14-823-480
Stepvial System™ Crimp/Snap Vial	Fisher Scientific	Cat#05-704-225
Xbridge BEH amide column (150 × 2.1 mm, 3 μm particle size)	Waters	Cat#186006724
DMEM	Gibco	Cat#11965118
Fetal Bovine Serum (FBS)	Sigma-Aldrich	Cat#F0926-500ML
Critical commercial assays
PureLink RNA Mini Kit	Ambion	Cat#12183018A
RNA Clean & Concentrator kit	Zymo Research	Cat#R1016
Experimental models: Cell line
Human: HEK293E	ATCC	Cat#293c18; RRID: CVCL_6974
Software
Xcalibur	Thermo Scientific	n/a
ProteoWizard	ProteoWizard	n/a
MAVEN	Elucidata	n/a
Other
Refrigerated centrifuge	Eppendorf	Cat#2231000655
Chemical Hood	Labconco	Cat#3746704
Magnetic Rack	Bio-Rad	Cat#1614916
Mini centrifuge	Benchmark Scientific	Cat#C1008-R
NanoDrop 2000c	Thermo Scientific	Cat#ND2000C
Thermomixer C	Eppendorf	Cat#5382000023
Dry heat block	Benchmark Scientific	Cat#BSH6000
Vortex	Benchmark Scientific	Cat#BV1003
Thermo Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer	Thermo Scientific	n/a
Vanquish UHPLC System	Thermo Scientific	n/a