| Component | Quantity |
|---|---|
| DNA to be digested | 1 μg |
| 10× CutSmart Buffer | 5 μL (1×) |
| NcoI-HF | 1 μL (20 units) |
| XhoI | 1 μL (20 units) |
| Nuclease-free water | Up to 50 μL |
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