| PCR cycling conditions | |||
|---|---|---|---|
| Steps | Temperature | Time | Cycles |
| Initial denaturation | 95°C | 30 s | 1 |
| Denaturation | 95°C | 5 s | 40 cycles |
| Annealing | 55°C | 15 s | |
| Final extension | 68°C | 10 s | 1 |
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