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| D10 medium (store at 4°C) | Final concentration | Amount |
|---|---|---|
| DMEM | - | 450 mL |
| Fetal bovine serum | 10% | 50 mL |
| Total | n/a | 500 mL |
| Quantitative-PCR MasterMix (store −20°C) | Final concentration | Amount (for 20 μL reaction) |
|---|---|---|
| KiCqStart SYBR green qPCR ReadyMix (2×) | 1× | 10 μL |
| Forward primer | 200 nM | Variable |
| Reverse primer | 200 nM | Variable |
| Nuclease-free water | Variable | add to 20 μL |
| Template | 5 ng/mL | Variable |
| Total | n/a | 20 μL |
