The interactions between the SRCR domains of SCARA1, MARCO, and SCARA5 with the modified LDL and VLDL are Ca2+dependent.A, ELISA data for the interactions of the SRCR domains of hSCARA1, hMARCO, or hSCARA5 with AcLDL in the presence of Ca2+ (2 mM) or EDTA (1 mM). B, ELISA data for the interactions of the SRCR domains of hSCARA1, hMARCO, or hSCARA5 with OxLDL in the presence of Ca2+ (2 mM) or EDTA (1 mM). C, ELISA data for the interactions of the SRCR domains of hSCARA1, hMARCO, or hSCARA5 with OxVLDL in the presence of Ca2+ (2 mM) or EDTA (1 mM). D, FACS data of the hSCARA1-transfected cells incubated with the modified LDL or VLDL in the presence of Ca2+ or EDTA. E, confocal fluorescent images of the hSCARA1 (with GFP-tag)-transfected cells incubated with the modified LDL or VLDL in the presence of EDTA (the scale bar represents 25 μm). F, FACS data of the hMARCO-transfected cells incubated with the modified LDL or VLDL in the presence of Ca2+ or EDTA. G, FACS data of the hSCARA5-transfected cells incubated with the modified LDL or VLDL in the presence of Ca2+ or EDTA. ELISA data are representative of three repeated experiments and presented as the mean ± SD. AcLDL, acetylated LDL; LDL, low-density lipoprotein; OxLDL, oxidized LDL; OxVLDL, oxidized VLDL; SRCR, scavenger receptor cysteine-rich; VLDL, very-low-density lipoprotein.