Figure 6.

Mutagenesis studies of the interactions between the SRCR domains of the SR-A members and the modified LDL and VLDL.A, FACS data of the WT or the mutant E443S of hSCARA1-transfected cells incubated with the unmodified or modified LDL in the presence of Ca²⁺. B, FACS data of the WT or the mutant E443S of hSCARA1-transfected cells incubated with the unmodified or modified VLDL in the presence of Ca²⁺. C, confocal fluorescent images of the mutant E443S of hSCARA1 (with GFP tag)-transfected cells incubated with the modified LDL or VLDL in the presence of Ca²⁺ (the scale bar represents 25 μm). D, FACS data of the mutant E486A or D419A/D420A of hSCARA5-transfected cells incubated with AcLDL, OxLDL, or OxVLDL in the presence of Ca²⁺. Mock represents nontransfected cells. E, FACS data of the mutant D458A/D459A of hSCARA5-transfected cells incubated with AcLDL, OxLDL, or OxVLDL in the presence of Ca²⁺. F, FACS data of the mutant D423A or D426A of hSCARA5-transfected cells incubated with AcLDL, OxLDL, or OxVLDL in the presence of Ca²⁺. AcLDL, acetylated LDL; LDL, low-density lipoprotein; OxLDL, oxidized LDL; OxVLDL, oxidized VLDL; SRCR, scavenger receptor cysteine-rich; VLDL, very-low-density lipoprotein.