Figure 8.

Interactions of SCARA3 and SCARA4 with lipoproteins and a model for lipoprotein recognition by the SR-A members.A, FACS data of the hSCARA3- or hSCARA4-transfected cells incubated with AcLDL or AcVLDL. Mock represents nontransfected cells. B, FACS data of the hSCARA3- or hSCARA4-transfected cells incubated with OxLDL or OxVLDL. C, ELISA data for the interactions of the CL region of hSCARA3 with AcLDL or OxLDL in the presence of Ca²⁺ (2 mM). The SRCR domain of SCARA1 was applied as a control. D, ELISA data for the interactions of the CL region of hSCARA3 with OxVLDL in the presence of Ca²⁺ (2 mM). E, FACS data of the hSCARA4-transfected cells incubated with the modified LDL or VLDL in the presence of Ca²⁺ or EDTA. F, a cartoon representation of the recognition of the modified LDL/VLDL by the SR-A members SCARA1, MARCO, and SCARA5. ELISA data are representative of three repeated experiments and presented as the mean ± SD. AcLDL, acetylated LDL; AcVLDL, acetylated VLDL; apoB, apolipoprotein B; CL, collagen-like; LDL, low-density lipoprotein; OxLDL, oxidized LDL; OxVLDL, oxidized VLDL; SRCR, scavenger receptor cysteine-rich; VLDL, very-low-density lipoproteins.