Antibodies |
|
Rabbit monoclonal anti-E-cadherin |
Cell Signaling Technology |
Cat#3195; RRID: AB_2291471
|
Rabbit polyclonal anti-β-Catenin |
Sigma |
Cat#C2206; RRID: AB_476831
|
Mouse anti-human LAMP1 |
BD Biosciences |
Cat#555798; RRID: AB_396132
|
Goat Alexa Fluor 568 anti-mouse secondary |
Life Technologies |
Cat#A-11031; RRID: AB_144696
|
Goat Alexa Fluor 488 anti-rabbit secondary |
Life Technologies |
Cat#A-11034; RRID: AB_2576217
|
Mouse monoclonal anti-GAPDH |
Santa Cruz |
Cat#sc-47724; RRID: AB_627678
|
Rabbit monoclonal anti-phospho-SAPK/JNK (Thr183/Tyr185) |
Cell Signaling Technology |
Cat#4668; RRID: AB_823588
|
Rabbit polyclonal anti-SAPK/JNK |
Cell Signaling Technology |
Cat#9252; RRID: AB_2250373
|
Rabbit polyclonal anti-p38 MAPK |
Cell Signaling Technology |
Cat#9212; RRID: AB_330713
|
Rabbit polyclonal anti-phospho-p38 MAPK (Thr180/Tyr182) |
Cell Signaling Technology |
Cat#9211; RRID: AB_331641
|
Mouse monoclonal anti-p53 |
Santa Cruz |
Cat#sc-126; RRID: AB_628082
|
|
Chemicals, peptides, and recombinant proteins |
|
Cell Tracker green |
Life Technologies |
Cat# C7025 |
Cell Tracker red |
Life Technologies |
Cat# C34552
|
Y-27632 |
Tocris Bioscience |
Cat# 1254 |
SP600125 |
Tocris Bioscience |
Cat#1496 |
SB202190 |
Cayman Chemical |
Cat#10010399 |
4′,6-Diamidino-2-Phenylindole (DAPI) |
Life Technologies |
Cat#1306 |
Trihydrochloride (Hoechst) |
Thermo Fisher Scientific |
Cat#3570 |
Z-VAD-FMK |
Sigma |
Cat#V116 |
Recombinant Murine IFN-γ |
Peprotech |
Cat#315-05 |
Concanamycin A |
Sigma |
Cat#C9705 |
|
Critical commercial assays |
|
Pierce BCA protein assay Kit |
Thermo Fisher Scientific |
Cat#23225 |
|
Experimental models: cell lines |
|
Human: MCF7 breast cancer cells |
Lombardi Cancer Center |
NA |
Human: BxPC3 pancreatic cancer cells |
ATCC |
Cat#CRL-1687 |
Human: HCT 116 colorectal cancer cells |
CLS |
RRID: CVCL_0291 |
Human: HCT116 p53 KO |
CLS |
RRID: CVCL_HD97 |
Mouse: J774.1 macrophages |
ATCC |
Cat#TIB-67 |
Human: 16HBE bronchial epithelial cells |
Sigma |
Cat#SCC150 |
Human: U-937 monocytes |
ATCC |
Cat#CRL-1593.2 |
Human: MCF7 JNKKTR |
Overholtzer laboratory |
This study |
Human: MCF7 shp53 |
Overholtzer laboratory |
This study |
|
Recombinant DNA |
|
Plasmid: pBabe-H2B-mCherry |
Overholtzer laboratory |
Florey et al. (2011) |
Plasmid: pLJM1-JNKKTR-mCardinal |
Albeck laboratory |
This study |
Plasmid: pLKO.1-shMAPK8 |
Sigma |
NM_139049 |
Plasmid: pLKO.1-shMAPK9 |
Sigma |
NM_139069 |
Plasmid: pLKO.1-shMAPK14 |
MSKCC core facility |
NM_001315 |
|
Software and algorithms |
|
NIS Elements software |
Nikon |
NA |
Volocity image analysis software |
PerkinElmer |
NA |
Prism 7 |
GraphPad |
NA |
|
Other |
|
35 mm glass-bottom dishes |
MatTek |
Cat# P06G-1.5-20-F |
12-Well glass-bottom dishes |
MatTek |
Cat# P12G-1.5-14-F |
Fetal bovine serum |
Sigma |
Cat#F2442 |