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. 2021 Jul 24;24(8):102902. doi: 10.1016/j.isci.2021.102902
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit monoclonal anti-E-cadherin Cell Signaling Technology Cat#3195; RRID: AB_2291471
Rabbit polyclonal anti-β-Catenin Sigma Cat#C2206; RRID: AB_476831
Mouse anti-human LAMP1 BD Biosciences Cat#555798; RRID: AB_396132
Goat Alexa Fluor 568 anti-mouse secondary Life Technologies Cat#A-11031; RRID: AB_144696
Goat Alexa Fluor 488 anti-rabbit secondary Life Technologies Cat#A-11034; RRID: AB_2576217
Mouse monoclonal anti-GAPDH Santa Cruz Cat#sc-47724; RRID: AB_627678
Rabbit monoclonal anti-phospho-SAPK/JNK (Thr183/Tyr185) Cell Signaling Technology Cat#4668; RRID: AB_823588
Rabbit polyclonal anti-SAPK/JNK Cell Signaling Technology Cat#9252; RRID: AB_2250373
Rabbit polyclonal anti-p38 MAPK Cell Signaling Technology Cat#9212; RRID: AB_330713
Rabbit polyclonal anti-phospho-p38 MAPK (Thr180/Tyr182) Cell Signaling Technology Cat#9211; RRID: AB_331641
Mouse monoclonal anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082

Chemicals, peptides, and recombinant proteins

Cell Tracker green Life Technologies Cat# C7025
Cell Tracker red Life Technologies Cat# C34552
Y-27632 Tocris Bioscience Cat# 1254
SP600125 Tocris Bioscience Cat#1496
SB202190 Cayman Chemical Cat#10010399
4′,6-Diamidino-2-Phenylindole (DAPI) Life Technologies Cat#1306
Trihydrochloride (Hoechst) Thermo Fisher Scientific Cat#3570
Z-VAD-FMK Sigma Cat#V116
Recombinant Murine IFN-γ Peprotech Cat#315-05
Concanamycin A Sigma Cat#C9705

Critical commercial assays

Pierce BCA protein assay Kit Thermo Fisher Scientific Cat#23225

Experimental models: cell lines

Human: MCF7 breast cancer cells Lombardi Cancer Center NA
Human: BxPC3 pancreatic cancer cells ATCC Cat#CRL-1687
Human: HCT 116 colorectal cancer cells CLS RRID: CVCL_0291
Human: HCT116 p53 KO CLS RRID: CVCL_HD97
Mouse: J774.1 macrophages ATCC Cat#TIB-67
Human: 16HBE bronchial epithelial cells Sigma Cat#SCC150
Human: U-937 monocytes ATCC Cat#CRL-1593.2
Human: MCF7 JNKKTR Overholtzer laboratory This study
Human: MCF7 shp53 Overholtzer laboratory This study

Recombinant DNA

Plasmid: pBabe-H2B-mCherry Overholtzer laboratory Florey et al. (2011)
Plasmid: pLJM1-JNKKTR-mCardinal Albeck laboratory This study
Plasmid: pLKO.1-shMAPK8 Sigma NM_139049
Plasmid: pLKO.1-shMAPK9 Sigma NM_139069
Plasmid: pLKO.1-shMAPK14 MSKCC core facility NM_001315

Software and algorithms

NIS Elements software Nikon NA
Volocity image analysis software PerkinElmer NA
Prism 7 GraphPad NA

Other

35 mm glass-bottom dishes MatTek Cat# P06G-1.5-20-F
12-Well glass-bottom dishes MatTek Cat# P12G-1.5-14-F
Fetal bovine serum Sigma Cat#F2442