Figure 6.

Effect of Ag-NPs on TERT and cyclin D1 protein and gene expression in cardiomyogenically differentiated BM-MSCs. Cells were cultured in 6-well plates at a concentration of 1 × 10⁶ cells/well. Protein and RNA were extracted from group I (cardiomyogenically differentiated BM-MSCs without Ag-NP treatment), group II (BM-MSCs in the presence of 2.5 µg/mL Ag-NPs), and group III (cardiomyogenically differentiated BM-MSCs in the presence of 2.5 µg/mL Ag-NPs) as described in the Experimental section and used for western blotting (A–C); (**P < 0.01 and ***P < 0.001), and real-time PCR (D and E); (*P < 0.05 and **P < 0.01), respectively.