Figure 8.

Effect of Ag-NPs on Wnt-3 and β-catenin protein and gene expression in cardiomyogenically differentiated BM-MSCs. (A–C) Protein and RNA were extracted from group I (cardiomyogenically differentiated BM-MSCs without Ag-NP treatment), group II (BM-MSCs in the presence of 2.5 µg/mL Ag-NPs), and III (cardiomyogenically differentiated BM-MSCs in the presence of 2.5 µg/mL Ag-NPs) as described in methods section and was subjected to western blotting; (*P < 0.05, **P < 0.01 and ****P < 0.0001) and real-time PCR (D and E); (*P < 0.05, **P < 0.01, and ***P < 0.001), respectively.