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. Author manuscript; available in PMC: 2021 Aug 10.
Published in final edited form as: Nature. 2018 Sep 26;562(7726):281–285. doi: 10.1038/s41586-018-0567-3

Figure 1. scMNase-seq measures simultaneously the position of both nucleosome and subnucleosome-sized particles in single cells.

Figure 1.

a, Schema of scMNase-seq. b, Plot of non-redundant nucleosome read number (x-axis) and genomic coverage of nucleosomes (y-axis) for NIH3T3, CD4 T and mES single cells. c, Average density profiles of nucleosomes (red) and subnucleosome-sized particles (blue) relative to TSS of active genes (left) and CTCF binding sites (right) for pooled CD4 T scMNase-seq data. d, Genome browser view of single-cell nucleosome positions for NIH3T3, CD4 T cell, and mESCs at TSSs of three representative cell type specific gene loci. Single cell libraries that have at least one nucleosome within any of three genomic regions were shown. Tracks for tag density of corresponding bulk cell MNase-seq data (one representative from two repeated experiments was shown) and pooled scMNase-seq data (all single cell libraries were included) were also shown. The nucleosome maps at expressed genes for each cell type were highlighted with pink rectangle. The expression levels of genes were shown in the heat map above the tracks.