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. 2021 Jul 26;22(4):1059. doi: 10.3892/etm.2021.10493

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of ALI induction on cell apoptosis, and the expression of miR-139-5p and ROCK1. (A) Histological analysis of mouse lung tissue samples using TUNEL staining. Representative histological images (magnification, x400) and percentage of positively stained cells. Scale bar, 100 µm. (B) miR-139-5p levels in lung tissues were assessed using RT-qPCR analysis. ROCK1 levels in lung tissues were assessed by (C) RT-qPCR and (D) western blotting. Data are presented as the mean ± standard deviation. ^**P<0.01, ^***P<0.001 vs. normal group. RT-qPCR, reverse transcription-quantitative PCR; ALI, acute lung injury; ROCK1, Rho-associated kinase 1; miR, microRNA.