Figure 5.

Effects of transfection with miR-139-5p mimics and Ov-ROCK1 on the expression of miR-139-5p and ROCK1 in LPS-stimulated NHBEs. (A) NHBEs were transfected with miR-NC mimics or miR-139-5p mimics and subsequently treated with LPS. The relative expression of miR-139-5p was examined using RT-qPCR. NHBEs were transfected with the empty vector control or Ov-ROCK1 and the expression levels of ROCK1 were analyzed using (B) RT-qPCR and (C) western blotting. Data are presented as the mean ± standard deviation. ^***P<0.001 vs. normal (control) group; ^*P<0.01 vs. LPS + miR-NC. ROCK1, Rho-associated kinase 1; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR; NHBEs, normal human bronchial epithelial cells; Ov, overexpression; LPS, lipopolysaccharide.