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Effect of hyperoxia on Rb, p-Rb and E2F1 expression. (A) Representative protein bands for Rb, p-Rb, E2F1 and the β-actin loading control. (a) Normoxia and (b) hyperoxia on postnatal day 3. (c) Normoxia and (d) hyperoxia on postnatal day 7. (e) Normoxia and (f) hyperoxia on postnatal day 14. (B) p-Rb/Rb ratio in LFs. (C) E2F1 protein expression in lung fibroblasts. n=5 for each experiment group. *P<0.05 and **P<0.01. p-, phosphorylated; Rb, retinoblastoma-associated protein.
