Fig. 1.

Editing of GUX1with a single guide RNA. A. Graphical representation of the GUX1 gene sequence showing the gRNA target sites and primer binding sites. B. Pattern of edits identified in the 15 somatic embryogenic tissue lines. C. Allelic variations identified in different plantlets. Edited plantlet line numbers are denoted in parenthesis. CRISPR gRNA sites are underlined. Red text denotes the PAM site, blue text denotes insertions and “-“ denotes deletions. ‘Y’ denotes a cytosine or thymine nucleotide. Scale bar 100 bp