FIGURE 6.

Protein arginine methyltransferase 5 (PRMT5) knockdown reverses protein kinase B (AKT) activation‐induced lipid production in lung adenocarcinoma cells. NCI‐H1299 cells with or without PRMT5 stable deletion were cultured in medium without FBS for 24 h. SC79 (4 µg/mL) was added (DMSO as control [Sh‐NC]) for 36 h, and intracellular lipids in the NCI‐H1299 cells were analyzed. A, Lipid metabolites were analyzed by gas chromatography‐mass spectrometry, and results were normalized by total protein levels. ^# P > .05; *P < .05; **P < .005. B, Triglyceride (TG) levels were quantitated and normalized by total protein levels and levels in control cells. C, Representative oil red O staining images. Scale bar = 50 µm