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. 2021 Jun 13;112(8):3302–3313. doi: 10.1111/cas.14982

FIGURE 1.

FIGURE 1

VLX1570 inhibits the proliferation of leukemia cells and induces apoptosis in most cell lines. (A) Eleven cell lines, including eight myeloid leukemia cell lines (HL‐60, MDS‐L, F‐36P, THP‐1, MOLM13, OCI‐AML3, MV4‐11, K562) and three lymphoid cell lines (MOLT‐4, Jurkat, U937), were cultured with VLX1570 (0‐100 nmol/L) for the indicated times. The cell count was measured by MTT assay. The value without VLX1570 was adjusted to 100%. The data represent the mean values with SD from five independent experiments. Solid line, 24 h; dotted line, 48 h. (B) These cell lines were cultured with different concentrations of VLX1570 (0‐100 nmol/L) for the indicated times and apoptosis was assessed by flow cytometry using annexin V‐FITC/PI staining. The single‐positive fraction for annexin V‐FITC (white bar) implies early apoptosis and the double‐positive fraction for annexin V‐FITC/PI (black bar) implies late apoptosis. The data represent the mean values with SD from three independent experiments. (C) Cell lysates of each cell line treated with or without 100 nmol/L of VLX1570 for 12 h were analyzed by immunoblotting analysis for detection of cleaved PARP (cPARP). The amount of β‐actin is shown as a loading control