FIGURE 3.

GSEA revealed the activation of the gene sets associated with HSF activation in VLX1570‐treated HL‐60 cells. (A) VLX1570 (50 nmol/L)‐treated or untreated HL‐60 cells were harvested at 3 h. Gene expression profiling was examined in triplicate experiments and obtained data were used for GSEA. The gene set “HSF1 activation”, “HSF1‐dependent transactivation”, and “Regulation of HSF1‐mediated heat shock response” were strongly upregulated by VLX1570 treatment and several statistical values are also presented. (B) Heat map presentation of affected genes included in the gene set is shown in the triplicate experiments (VLX1570 50 nmol/L: 50 nmol/L of VLX1570‐treated; control: untreated)