FIGURE 2.

Effect of fucosyltransferase 8 (FUT8) expression on cell migration and proliferation. FUT8 expression in normal breast epithelial (MCF10A) and breast cancer (BC) cells (MCF7, MDA‐MB‐231, MDA‐MB453) assessed by Western blotting (A) and quantitative real‐time PCR (qRT‐PCR) (B). Two FUT8‐stable transfectants of MCF10A (FUT8 OE#1, FUT8 OE#2) and their vector blank (control) were established and assessed by Western blotting (C), qRT‐PCR (D), and immunofluorescence staining (E). F, Migration ability of FUT8‐overexpressing MCF10A and vector control assessed by transwell migration without Matrigel. Two FUT8‐knockdown MDA‐MB‐231 transfectants (FUT8 sh#1, FUT8 sh#2) and their vector blank (shNTC) were established and assessed by Western blotting (G), qRT‐PCR (H), and immunofluorescence staining (I). Cell proliferation (J), scratch‐wound assay (K), and transwell invasion assay (L) of FUT8‐knockdown MDA‐MB‐231