FIGURE 3.

Fucosyltransferase 8 (FUT8) enhances doxorubicin (DOX) susceptibility (in vitro and in vivo) and stemness of MDA‐MB‐231. A, Survival ratio of two FUT8‐knockdown MDA‐MB‐231 lines, vector blank (vector), and wild‐type cells (231WT) following 24, 48, or 72 h treatment with 0, 5, 10, 15, or 20 μM DOX. B, In vivo xenograft experiment (schematic). C, Tumor growth curve for BALB/c‐nu mice injected with vector or FUT8 sh#1 cells, and treated with 0.9% normal saline (vector + 0.9%NS, FUT8 sh#1 + 0.9%NS) or with DOX (vector + DOX, FUT8 sh#1 + DOX). n = 4 for each group. D, Isolated tumors of xenograft models and tumor weights for various groups. E, Representative H&E and immunohistochemical (IHC) staining images of s.c. tumors of FUT8, Ki‐67, and TUNEL in various groups. F, Percentage of CD44⁺/CD24⁻ cells, assayed by flow cytometry. G, Upper: representative image of spheres formed by vector or FUT8 sh#1 cells. Lower: number of spheres per well