FIGURE 4.

MiR‐10b modulates fucosyltransferase 8 (FUT8) expression via AP‐2γ. A, Venn diagram of miR‐10b target genes predicted by three web servers (TargetScan, miRDB, TarBase). B, Correlation of expression between FUT8 and NR6A1, or AP‐2γ. Data presented as log2 fold change. MCF10A (C) and MDA‐MB‐231 (D) were treated with miR‐10b mimic, and expression of FUT8 and AP‐2γ was assessed by Western blotting. Right panel shows quantitation of representative images. E, Putative miR‐10b binding sites on wild‐type sequence and mutant sequence of AP‐2γ 3'‐UTR. F, MCF10A cells were cotransfected with miR‐10b mimic or mimic‐nc and two reporter plasmids psiCHECK2 (wild‐type or mutant AP‐2γ 3'‐UTR sequence), and luciferase activities of transfectant cells were assayed. G, Same as panel F but with MDA‐MB‐231