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. 2021 Jun 12;112(8):3190–3204. doi: 10.1111/cas.14987

FIGURE 6.

FIGURE 6

Fucosyltransferase 8 (FUT8) is regulated by AP‐2γ via STAT3. A, STAT3, p‐STAT3, AP‐2γ, and FUT8 expression in MCF10A, MDA‐MB‐231, MDA‐MB‐468, and MDA‐MB‐453. B, FUT8, STAT3, and p‐STAT3 expression in AP‐2γ OE/231. C, STAT3, p‐STAT3, AP‐2γ, and FUT8 expression in AP‐2γ–knockdown MDA‐MB‐468 (siAP‐2γ‐1/468, siAP‐2γ‐2/468). D, STAT3, p‐STAT3, and FUT8 expression in MDA‐MB‐231 treated for 48 h with various concentrations of JSI‐124 (see Results/Section 3.6: "Regulatory effect of AP‐2γ…"). E, MDA‐MB‐231 lysate was subjected to immunoprecipitation with antibodies directed to AP‐2γ, STAT3, and p‐STAT3. F, Upper: FUT8 promoter region (schematic) indicating Jaspar‐predicted binding site of p‐STAT3, promoter regions including ChIP PCR primers (A, B, C), and transcription start site (TSS). Lower: ChIP PCR results, using three sets of different primers