FIGURE 2.

Epigenetic alteration of enhancers in MKN7 after Epstein‐Barr virus (EBV) infection. A, Peak finding analysis in MKN7_EB compared with MKN7_WT. Comparison of active mark H3K27ac; a total of 8310 regions with >2‐fold increase were extracted. B, Classification of H3K27ac increased peaks. WT, MKN7_WT; EB, MKN7_EB. H3K27ac increased peaks are classified using the promoter mark H3K4me3 and the enhancer mark H3K4me1. C, Expression of genes nearest to each activated enhancer was significantly upregulated in MKN7_EB. D, Enriched de novo motif at nuclear‐free regions predicted by HOMER from H3K27ac data. E, RNA expression levels in best‐matched transcription factors (TFs). EHF was strongly activated in MKN7_EB. F, RNA expression levels of EHF in The Cancer Genome Atlas (TCGA) data for different gastric cancer (GC) subtypes. G, IHC of EHF (top) and H&E staining (bottom). H, immunohistochemistry (IHC) scores of EHF were higher in EBV‐positive GC than EBV‐negative GC and normal tissues