FIGURE 1.

Cyclin‐dependent kinase‐like 3 (CDKL3) is upregulated in glioma and correlates with prognosis. A, RNA sequencing heatmap of glioma and normal tissues (3 v 3) that identified 3469 differentially expressed genes, including 1652 upregulated genes and 1817 downregulated genes. B, Expression of CDKL3 in tumor and normal tissues was detected by immunohistochemistry analysis. C, Immunohistochemistry staining score of CDKL3 expression in 128 glioblastoma cases is significantly higher than that in 16 normal brain tissues (P < .001). CDKL3 expression is positively related to pathological grade of glioma (P < .001). D, Data collected from the GSE15824 dataset shows the upregulated expression of CDKL3 in glioma cells compared with normal astrocytes. E, CDKL3 expression correlates with survival time of glioblastoma patients. Kaplan‐Meier survival analysis in 128 GBM cases stratified by CDKL3 expression indicates that high level of CDKL3 expression was associated with shorter overall survival (10 vs 15 months, P = .0052). F, G, Knockdown efficiency of CDKL3 in U87 and U251 cells was evaluated by (F) quantitative PCR and (G) western blot analysis. Data are presented as mean ± SD. ***P < .001. shCtrl, normal control