FIGURE 3.

Knockdown of cyclin‐dependent kinase‐like 3 (CDKL3) inhibits cell migration. A, B, Inhibition effect of CDKL3 on glioma cell migration was confirmed by (A) wound healing assay and (B) Transwell assay. C, Western blot (WB) analysis was used to detect the expression of epithelial‐mesenchymal transition‐related proteins including E‐cadherin, vimentin, and Snail in U87 and U251 cells. D, WB analysis was utilized to detect the expression of stemness‐ and inflammation‐related proteins including CD133, MELK, SOX2, Toll‐like receptor 4 (TLR4), nuclear factor‐κB (NF‐κB), and c‐Myc in U87 cells. Representative images were selected from at least three independent experiments. Data are presented as mean ± SD. **P < .01, ***P < .001. shCtrl, normal control