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. Author manuscript; available in PMC: 2022 Aug 3.
Published in final edited form as: Cell Metab. 2021 Jul 15;33(8):1671–1684.e4. doi: 10.1016/j.cmet.2021.06.012

Figure 5. Decreased intestinal lipid absorption requires FXR-dependent changes in bile acids.

Figure 5.

(A) Experiment schematic: wild-type or Fxr−/− mice (n = 11–12/group) fed a standard rodent diet were treated for 3 days with vehicle or FXR agonist GSK2324.

(B) Fecal fatty acids in mice from (A).

(C and D) Total biliary bile acid concentration (C) and biliary bile acid composition (D) in mice from (A).

(E) Experiment schematic: wild-type mice fed either a standard rodent diet or a diet supplemented with cholic acid were treated with vehicle or GSK2324 for3 days (n = 10/group).

(F) Hepatic mRNA expression of Shp and MafG in mice from (E).

(G–J) Total biliary bile acid concentration (G) and biliary bile acid composition (H) in mice from (E). Lipidomic analyses of (I) total hepatic TAG and (J) individual hepatic TAG species (nmol/mg liver) in mice from (E).

(K) Fecal fatty acids in mice from (E).

Data are represented as mean ± SEM with individual animals noted as dots. *p < 0.05, ***p < 0.001. See also Figure S5.