| Biological samples |
|
|
| Human liver biopsies |
Al-Dury et al., 2019
|
N/A |
| Chemicals, peptides, and recombinant proteins |
|
|
| Sucrose polybehenate (SPB) |
CarboMer |
Cat # 56449-50-4 |
| Deuterium oxide |
Sigma-Aldrich |
Cat # 151882 |
| Bile acid standards |
See Table S3
|
N/A |
| Fatty Acid Standards |
Nu-Chek-Prep |
GLC96, GLC20a, GLC96b, GLC96c |
| Experimental models: Organisms/strains |
|
|
|
Fxr−/− mice (whole-body) |
Jackson Laboratory |
Cat# JAX 004144 |
|
Shp−/− mice |
Kind gift from Dr. Bryan Goodwin |
N/A |
|
Srebp1c−/− mice |
Jackson Laboratory |
Cat# JAX 004365 |
|
Pparα−/− mice |
Jackson Laboratory |
Cat# JAX 008154 |
| Albumin-Cre mice |
Jackson Laboratory |
Cat# JAX 003574 |
| Villin-Cre mice |
Jackson Laboratory |
Cat# JAX 021504 |
|
Fxr
fl/fl
|
Jackson Laboratory |
Cat# JAX 028393 |
| Oligonucleotides |
|
|
| Primer sequences for qPCR |
See Table S1
|
N/A |
| Software and algorithms |
|
|
| MsAnalyzer (Custom software for GCMS Analysis) |
https://github.com/gcalmettes/labUtils
|
N/A |
| Other |
|
|
| Standard rodent chow |
Purina |
Cat# 5053 |
| Western diet |
Research Diets |
Cat# D12079B |
| Standard rodent chow with 0.8% SPB |
Envigo |
Cat# TD170975 |
| Standard rodent chow with 0.8% SPB and 0.5% CA |
Envigo |
Cat# TD170976 |
| Western diet with 1.04% SPB |
Research Diets |
Cat# D17121803 |
