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. Author manuscript; available in PMC: 2021 Aug 10.
Published in final edited form as: FASEB J. 2020 Jul 30;34(9):12239–12254. doi: 10.1096/fj.202000017RR

Figure. 5.

Figure. 5

Pitx3-A53T α-Syn × Tau−/− mice specifically developed severe impairment of MAP1A in the SNR. (A,B) Western blot showed the expression levels of MAP1A in the midbrain homogenates of 2- and 12-month-old mice, respectively. (C) Western blot showed the expression levels of MAP1A in the midbrain homogenates of 18-month-old nTg and Tau−/− mice. (D) Bar graph represented the expression levels of MAP1A in the midbrains of 2-, 6-, 12- and 18-month-old mice. Values are mean ± SEM. *P < 0.05 (12- and 18-month-old versus 6-month-old Pitx3-A53T α-Syn × Tau−/−). (E,F) PSD-95 (green), MAP1A (red) and PV (blue) costaining in the SNR of 2- and 12-month-old mice, respectively. Arrowheads marked PSD-95 staining; arrows pointed to MAP1A staining; asterisks indicated PV+ neurons. Scale bar: 10 μm. n = 5 per genotype per time point.