Figure 8.
Summary of data for compound 41 (CCF0058981). Images show antiviral activity of 41 and remdesivir against SARS-CoV-2 by plaque reduction assay. Vero E6 ACE2 cells were infected with approximately 50 plaque forming units per well of SARS-CoV-2. After 1 h incubation, the viral inoculum was removed, and the cells were overlaid with DMEM containing 1% low-melting agarose and indicated concentration of test compounds. At 3 days post-infection, the cells were fixed with 4% formaldehyde for at least 1 h. Overlay was then removed and cells were stained with 0.2% crystal violet containing 20% ethanol. The images are representatives of two repeats. Tier 1 DMPK data were obtained at Q2 Solutions.