Figure 3.

Effect of metformin on the open-field locomotor activity of SCI mice.

Photobeam Activity System open-field locomotor function test was performed 4 weeks post injury. (A) The test chamber of the PAS system. (B–E) Rearing activity (B), rest time (C), average speed (D) and travel distance (E) were shown by scatter plots. Data are presented as the mean ± SEM (n = 5 in control, n = 7 in 0 dpi, n = 8 in 3 dpi). *P < 0.05, vs. control group; #P < 0.05, vs. 0 dpi group (one-way analysis of variance followed by Bonferroni post hoc test). Control: Vehicle treated; 0 dpi or 3 dpi: the first dose of metformin given immediately or 3 days post SCI, respectively. SCI: Spinal cord injury.