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. 2021 Mar 25;16(11):2257–2263. doi: 10.4103/1673-5374.310697

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NEUROD1-induced reactive astrocytes at 7 days after infection (immunofluorescence staining).

(A) NEUROD1 overexpression led to significantly increased DCX immunopositivity (red). (B) Percentage of DCX positive cells. Scale bar: 10 µm. Data are expressed as the mean ± SD. The experiment was repeated three times. **P < 0.01 (unpaired Student’s t-test). BF: Bright field; DAPI: 4′,6-diamidino-2-phenylindole; DCX: doublecortin; GFP: green fluorescent protein; NEUROD1: neurogenic differentiation factor 1; NV: non-virus group.