Figure 4.

Effects of SPIO-PLL-labeled BMSC transplantation on pathological damage in brain tissue of HIBD rats (hematoxylin/eosin staining).

Compared with the sham group, more extensive cerebral edema (red arrows) and inflammatory responses (blue arrows) were observed in the brains of HIBD rats from week 1 to week 4 after the operation. After SPIO-PLL-labeled BMSC injection, the degree of edema and cell death was reduced, and at week 4 after treatment, the degree of cerebral edema was significantly reduced, and cell apoptosis was barely observed. Moreover, the intraventricular injection of SPIO-PLL-labeled BMSCs showed better effects for the promotion of recovery from these pathological changes. Scale bars: 200 µm. BMSCs: Bone marrow-derived mesenchymal stem cells; HIBD: hypoxic-ischemic brain damage; ic.: intraventricular injection; MG: magnetic guidance; PLL: poly L-lysine; SPIO: superparamagnetic iron oxide nanoparticles; tv.: tail vein injection.