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. 2021 Jul 27;12:699665. doi: 10.3389/fpls.2021.699665

FIGURE 3.

FIGURE 3

Stability of NPC-2 by IC-RT-PCR. Amplification of an 820-bp fragment, which is the size expected for the recombinant clone, was observed in 100% of the plants infected. MW, hiperladder 200 bp; TMV, empty vector, C1, TMV-PSN-NPC2 clone (820 pb). 1–8, Infected plant, C2, TMV-PSN-EGF clone (397 pb), BL1, negative PCR control; C3, infected plant with TMV-PSN-EGF (IC-RT-PCR positive control). HP: healthy plant, BL2: negative control of IC-RT-PCR. (A) Infected plants to 8 dpi and (B) infected plants to 12 dpi.