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. 2021 Jul 27;11:718731. doi: 10.3389/fonc.2021.718731

Figure 2.

Figure 2

miRNA and mRNA alteration of BM LSKs in Scl/tTA-BA mice. (A) Number of dysregulated miRNAs detected by miRNA array (fold-change ≥1.5 or ≤0.67). (B) Normalized signal values of representative differentially expressed miRNAs. (C) Expression detection of BA in BM LSKs of Scl/tTA-BA mice. (D) qRT-PCR confirmation of expression level of selected miRNAs in BM LSKs from Scl/tTA-BA mice. (E) Expression detection of BA in 32D-BA cell line. (F) qRT-PCR confirmation of expression level of selected miRNAs in 32D-BA cell line. (G) The number of dysregulated genes detected by RNA-seq (fold-change≥2 or ≤0.5). (H, I) GO analysis of differentially expressed genes between BA-harboring and normal BM LSKs. (J) GSEA showing that the apoptosis pathway was remarkably downregulated in BM LSKs at week-3 after doxycycline withdrawal. Results are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 (C–F) was assessed by unpaired t-test.