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. 2021 Jul 27;11:718731. doi: 10.3389/fonc.2021.718731

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Copyright © 2021 Chen, Xie, Liu, Liu, Li, Zhang, Zhang, Wang, Zhang, Sun and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phosphorylated (P) ERK inhibited the expression of miR-142a in BA-harboring cells. (A) Western blot detection of total and p-ERK protein level in 32D-BA and 32D-mCherry cells. (B) The ERK inhibitor SCH772984 inhibited the p-ERK level in 32D-BA cell line. (C) qRT-PCR detection of miR-142a-5p and miR-142a-3p level in 32D-BA cells with ERK inhibitor treatment. (D) qRT-PCR and Western blot analysis of Ciapin1 expression in 32D-BA cells with ERK inhibitor treatment. (E) Apoptosis analysis of 32D-BA cells with ERK inhibitor treatment. (F) Apoptosis analysis of 32D-BA-Ciapin1 cells with ERK inhibitor treatment. Results are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 (C–F) was assessed by unpaired t-test.