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. Author manuscript; available in PMC: 2021 Aug 10.
Published in final edited form as: Cell Rep. 2020 Sep 15;32(11):108142. doi: 10.1016/j.celrep.2020.108142

Figure 5. BV01 Alters Host Interactions with Bile Acids in a tspO-Dependent Manner.

Figure 5.

A) Representative LC-MS traces showing that B. vulgatus deconjugates taurocholic acid (TCA) to cholic acid (CA) in the cured lysogen background (ΔBV01), but little or no CA is detectable in the WT or cured lysogen tspO deletion (ΔBV01ΔtspO) backgrounds. B. vulgatus cultures were incubated with 50 μM TCA for 16 h prior to bile acid extraction.

(B) Representative LC-MS traces showing that B. vulgatus deconjugates glycocholic acid (GCA) to CA in the ΔBV01 background but not in the WT or ΔBV01ΔtspO backgrounds. B. vulgatus cultures were incubated with 50 μM GCA for 48 h prior to bile acid extraction. Nordeoxycholic acid (norDCA) was added to a final concentration of 15 μM as an internal standard after incubation. Peaks were labeled for their metabolites based on m/z; TCA, 514.29; GCA, 464.30; CA, 407.28; norDCA, 377.27.

(C) Areas under the curve from extracted ion chromatograms were used to calculate relative intensity and averaged for CA:TCA (n = 3) and CA:GCA (n = 4) ratios. Statistical analyses were performed with one-way ANOVA; CA:TCA df = 2, F = 6.59, p = 0.03; CA:GCA df = 3, F = 103.46, p < 0.0001. Asterisks illustrate the Tukey’s honestly significant difference test p value: *p < 0.05, **p < 0.01.