FIG 7.
Bystander senescence induces endothelial adhesiveness. Expression of adhesion molecules, VCAM-1 and ICAM-1, was analyzed by Western blot from TMNK-1 and EAhy926 cell lysates prepared after 24 h of exposure of CM from spike-expressing A549 cells with or without tocilizumab or zanubrutinib treatment (A and B). The blot in panel A was spliced to remove an extra lane, indicated by the black tooling line. The expression level of actin in each lane is shown as a total protein load control for comparison, and the background in the blot is decreased for clarity of the image. A comparative analysis of leukocyte (THP-1 cells were used as a model cell line for leukocytes) adhesion on endothelial cells (TMNK-1 and EAhy926) after 24-h exposure of CM in the presence or absence of tocilizumab or zanubrutinib is shown (C and D). Experiments were performed in triplicate, and results are presented as mean ± standard deviation. **, P < 0.005.