TABLE 2.

Characteristics of HIV-1 glycan and loop lengths in circulating sequences^a

Subtype	Env PNGS	V1 PNGS	V1 length	V2 PNGS	V2 length	V3 PNGS	V3 length	V4 PNGS	V4 length	V5 PNGS	V5 length
Subtype A1
y8090	28	4	29	2	43	1	35	4	32	1	14
y2000	29	4	29	2	42	1	35	5	32	2	14
y2005	29	4	32	2	42	1	35	5	32	1	14
y2010	29	4	31	2	42	1	35	5	33	1	14
K-W P	0.317	0.038	0.031	0.886	0.360	0.161	0.583	0.999	0.763	0.370	0.679
Subtype B
y8090	30	4	34	2	39	1	35	4	31	1	15
y2000	31	4	35	2	40	1	35	4	31	2	15
y2005	31	4	34	2	40	1	35	5	32	2	15
y2010	30	4	33	2	41	1	35	4	32	2	15
K-W P	0.315	0.333	0.009	0.0005	<0.0001	0.088	<0.0001	0.037	0.001	0.012	0.283
Subtype C
y8090	29	4	29.5	2	42	1	35	4	29	1	15
y2000	29	4	31.5	2	41	1	35	4	28	1	15
y2005	29	4	31	2	42	1	35	4	28	1	15
y2010	29	4	31	2	42	1	35	4	29	1	15
K-W P	0.047	0.063	0.003	0.602	0.209	0.644	0.004	0.186	0.001	0.007	0.715
Subtype D
y8090	29	4	28	2	41	1	34	4	31	2	14
y2000	29	4	34	2	40	1	34	4	31	2	15
y2005	28	3	29	2	40	1	34.5	4	30.5	2	15
y2010	28	4	29.5	2	39.5	1	34.5	5	32.5	1	14
K-W P	0.457	0.017	0.022	0.220	0.452	0.788	0.226	0.356	0.701	0.576	0.334
Subtype CRF01_AE
y8090	29	5	34	2	40	1	35	4	28	2	14
y2000	29	5	34	2	41	1	35	4	28	2	14
y2005	29	5	35	2	41	1	35	4	28	2	14
y2010	29	5	34	2	41	1	35	4	27	2	14
K-W P	0.813	0.363	0.263	0.641	0.320	0.018	0.210	0.282	0.022	0.315	0.920

^aK-W P, Kruskal-Wallis P value. Median values for loop lengths (expressed as the numbers of amino acids) are provided for circulating sequences for different time periods and subtypes or CRF. Kruskal-Wallis tests were used to compare the difference across time periods.