FIG 4.

Phylogenetic relationship of phiNY. (A) Phylogenetic relationships between glycoside hydrolases and lysozymes from bacteria and phages, which have similarity with ORF17 of phage phiNY. The name of the phage discovered in this study, phiNY, is shown in black. The names of bacterium reference sequences and phage reference sequences, including both the GenBank accession number and species name, are shown in blue and red, respectively. The cell wall hydrolase of Bacillus phage BCD7 is selected as an outgroup and is shown in green. (B) Phylogenetic analysis of RNA-dependent RNA polymerases from phiNY and other dsRNA phages. The phylogenetic tree is constructed based on RNA-dependent RNA polymerase protein alignment. The name of the phage discovered in this study, phiNY, is shown in black. The names of reference sequences in the genus Cystovirus and unclassified Cystoviridae species, including both the GenBank accession number and the phage species name, are shown in red and blue, respectively. Wenling crustacean virus 9 of Bunyavirales, which also has a segmented genome, is selected as an outgroup and is shown in green.