FIG 2.

Features of the grouper PABP protein. (A) The nucleotide and deduced amino acid sequences of the orange-spotted grouper (Epinephelus coioides) polyadenylate binding protein (PABP) gene were obtained from RT-PCR and next-generation sequencing (NGS) of grouper brain (GB) cells. Lowercase, untranslated nucleotides; uppercase, open reading frame nucleotides; bold uppercase, amino acid; yellow shading, RNA recognition motif 1 (RRM1); green shading, RRM2; blue shading, RRM3; orange shading, RRM4; red shading, C-terminal helical domain (PABC); underlined amino acids, proline-rich linker; asterisk, stop codon. (B) Schematic representation of the PABP domain structure and the amino acid sequence comparison among grouper, human, and yeast. Accession numbers for each PABP sequence are given in parentheses.