FIG 4.

Linc-Pint reduces DDX24 protein levels in the presence or absence of HCV. (A) Huh7.5 and Rep2a cells were transfected with control (empty vector) or pcDNA3 Linc-Pint plasmid DNA. Cell lysates were analyzed for DDX24 expression by Western blotting using a specific antibody. The blot was reprobed with antibody to actin for comparison of protein loads. Densitometric scanning results are presented as fold changes in a bar diagram at the bottom. (B) Lysates from Huh7.5 and Rep2a (Huh7.5 cells harboring a genome-length HCV replicon) cells were analyzed for DDX24 expression by Western blotting. The blot was reprobed with antibody to actin for comparison of protein loads. Densitometric scanning results are presented as fold changes in a bar diagram at the bottom. (C) Huh7.5 cells were transfected with the empty vector control (middle lane) or the pcDNA3 Linc-Pint plasmid (right lane) and then infected with HCV JFH1 (MOI = 1.0). Mock-infected cell lysates were used as the control. Cell lysates were analyzed for DDX24 expression by Western blotting using a specific antibody. The blot was reprobed with antibody to actin for comparison of protein loads. Densitometric scanning results are presented as fold changes in a bar diagram at the bottom. Data are presented as means ± SD from three independent experiments. Statistical significance was analyzed using two-tailed Student’s t test. *, P < 0.05; **, P < 0.01.